Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Local clustering of transferrin receptors promotes clathrin-coated pit initiation

Allen P. Liu, François Aguet, Gaudenz Danuser and Sandra L. Schmid
The Journal of Cell Biology
Vol. 191, No. 7 (DECEMBER 27, 2010), pp. 1381-1393
Stable URL: http://www.jstor.org/stable/25765637
Page Count: 13
  • More info
  • Cite this Item
Local clustering of transferrin receptors promotes clathrin-coated pit initiation
Preview not available

Abstract

Clathrin-mediated endocytosis (CME) is the major pathway for concentrative uptake of receptors and receptor—ligand complexes (cargo). Although constitutively internalized cargos are known to accumulate into maturing clathrin-coated pits (CCPs), whether and how cargo recruitment affects the initiation and maturation of CCPs is not fully understood. Previous studies have addressed these issues by analyzing the global effects of receptor overexpression on CME or CCP dynamics. Here, we exploit a refined approach using expression of a biotinylated transferrin receptor (bTfnR) and controlling its local clustering using mono- or multivalent streptavidin. We show that local clustering of bTfnR increased CCP initiation. By tracking cargo loading in individual CCPs, we found that bTfnR clustering preceded clathrin assembly and confirmed that bTfnR-containing CCPs mature more efficiently than bTfnR-free CCPs. Although neither the clustering nor the related changes in cargo loading altered the rate of CCP maturation, bTfnR-containing CCPs exhibited significantly longer lifetimes than other CCPs within the same cell. Together these results demonstrate that cargo composition is a key source of the differential dynamics of CCPs.

Page Thumbnails

  • Thumbnail: Page 
1381
    1381
  • Thumbnail: Page 
1382
    1382
  • Thumbnail: Page 
1383
    1383
  • Thumbnail: Page 
1384
    1384
  • Thumbnail: Page 
1385
    1385
  • Thumbnail: Page 
1386
    1386
  • Thumbnail: Page 
1387
    1387
  • Thumbnail: Page 
1388
    1388
  • Thumbnail: Page 
1389
    1389
  • Thumbnail: Page 
1390
    1390
  • Thumbnail: Page 
1391
    1391
  • Thumbnail: Page 
1392
    1392
  • Thumbnail: Page 
1393
    1393