Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Molecular Determination of Genetic Variation in Pisolithus Isolates from a Defined Region in New South Wales, Australia

I. C. Anderson, S. M. Chambers and J. W. G. Cairney
The New Phytologist
Vol. 138, No. 1 (Jan., 1998), pp. 151-162
Published by: Wiley on behalf of the New Phytologist Trust
Stable URL: http://www.jstor.org/stable/2588630
Page Count: 12
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Molecular Determination of Genetic Variation in Pisolithus Isolates from a Defined Region in New South Wales, Australia
Preview not available

Abstract

Sixty-two isolates of Pisolithus were obtained from a carpophore population collected from a 90-km$^2$ area of New South Wales, Australia. Random amplified polymorphic DNA (RAPD) analysis was used initially to screen isolates for variation, and restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer (ITS) region and ITS sequence analysis were subsequently performed on selected isolates. ITS-RFLP and ITS sequence analysis indicated that two isolates (LJ07 and WM01) from different sites were considerably different from all other isolates, a strict consensus tree generated by bootstrap analysis of sequence data strongly suggesting that isolates LJ07 and WM01 represent a separate Pisolithus species. Although the general morphology of carpophores and basidiospores of the majority of collected isolates was consistent with previous descriptions of P. tinctorius (Pers.) Coker and Couch, basidiospore characteristics suggest that isolates LJO7 and WMO1 might represent a Pisolithus species distinct from P. tinctorius and the previously described Australian species, P. microcarpus (Cooke & Massee) G. Cunn.

Page Thumbnails

  • Thumbnail: Page 
[151]
    [151]
  • Thumbnail: Page 
152
    152
  • Thumbnail: Page 
153
    153
  • Thumbnail: Page 
154
    154
  • Thumbnail: Page 
155
    155
  • Thumbnail: Page 
156
    156
  • Thumbnail: Page 
157
    157
  • Thumbnail: Page 
158
    158
  • Thumbnail: Page 
159
    159
  • Thumbnail: Page 
160
    160
  • Thumbnail: Page 
161
    161
  • Thumbnail: Page 
162
    162