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Identification and Some Biochemical Properties of the Major XBL Gene Product of Bovine Leukemia Virus
Noriyuki Sagata, Junko Tsuzuku-Kawamura, Mariko Nagayoshi-Aida, Fumio Shimizu, Ken-Ichi Imagawa and Yoji Ikawa
Proceedings of the National Academy of Sciences of the United States of America
Vol. 82, No. 23 (Dec. 1, 1985), pp. 7879-7883
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/26482
Page Count: 5
You can always find the topics here!Topics: Antiserum, Cell lines, Cell nucleus, Viruses, RNA, Messenger RNA, Bovine leukemia virus, Virions, Open reading frames, Cellular metabolism
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Using a rabbit antiserum directed against a synthetic oligopeptide whose sequence was deduced from the nucleotide sequence of the XBL gene of bovine leukemia virus, we detected a 38-kDa protein in virus-producing cell lines. In vitro translation of hybrid-selected RNA unequivocally demonstrates that this protein, designated p38(XBL), is indeed encoded by the XBL gene. Unlike the other virus-encoded proteins, however, p38(XBL) resides within the cells without being incorporated into virions. It undergoes no gross post-translational modifications and has a relatively short half-life (5-6 hr) in vivo. Furthermore, cell fractionation combined with pulse--chase experiment reveals that a significant fraction (more than half) of the p38(XBL) localizes to the nucleus of the infected cell after synthesis. We conclude that the XBL gene of bovine leukemia virus is a functional gene encoding a nonvirion protein p38(XBL), which possibly functions within the nucleus of the infected cell to regulate viral or cellular gene expression. p38(XBL) is presumably translated from a doubly spliced, bicistronic mRNA that has the capability to encode another small polypeptide in a different reading frame.
Proceedings of the National Academy of Sciences of the United States of America © 1985 National Academy of Sciences