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Scintillation Proximity Radioimmunoassay Utilizing 125I-Labeled Ligands

Sidney Udenfriend, Louise Diekmann Gerber, Larry Brink and Sydney Spector
Proceedings of the National Academy of Sciences of the United States of America
Vol. 82, No. 24 (Dec. 15, 1985), pp. 8672-8676
Stable URL: http://www.jstor.org/stable/26653
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Scintillation Proximity Radioimmunoassay Utilizing 125I-Labeled Ligands
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Abstract

A unique type of radioimmunoassay is described that does not require centrifugation or separation. Microbeads containing a fluorophor are covalently linked to antibody. When an 125I-labeled antigen is added it binds to the beads and, by its proximity, the emitted short-range electrons of the 125I excite the fluorophor in the beads. The light emitted can be measured in a standard scintillation counter. Addition of unlabeled antigen from tissue extracts displaces the labeled ligand and diminishes the fluorescent signal. Application of scintillation proximity immunoassay to tissue enkephalins, serum thyroxin, and urinary morphine is described. Applications of the principle to study the kinetics of interaction between receptors and ligands are discussed.

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