You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Bacteriophage T4 DNA Topoisomerase Mediates Illegitimate Recombination in vitro
Proceedings of the National Academy of Sciences of the United States of America
Vol. 83, No. 4 (Feb. 15, 1986), pp. 922-926
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/27066
Page Count: 5
You can always find the topics here!Topics: DNA, Bacteriophages, Enzymes, Crossovers, Recombinant DNA, Molecules, Maps, Monomers, Genomes, Coordinate systems
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
We have found that purified T4 DNA topoisomerase promotes recombination between two phage λ DNA molecules in an in vitro system. In this cross, T4 DNA topoisomerase alone is able to catalyze the recombination and produce a linear monomer recombinant DNA that can be packaged in vitro. ATP is not required for this recombination, while oxolinic acid stimulates it. The recombinant DNA molecules contain duplications or deletions, and the crossovers take place between nonhomologous and nonspecific sequences of λ DNA. Therefore, the recombination mediated by the T4 DNA topoisomerase is an illegitimate recombination that is similar to that mediated by Escherichia coli DNA gyrase. A model was proposed previously in which DNA gyrase molecules that are bound to DNA associate with each other and lead to the exchange of DNA strands through the exchange of DNA gyrase subunits. This model is also applicable to the recombination mediated by T4 DNA topoisomerase.
Proceedings of the National Academy of Sciences of the United States of America © 1986 National Academy of Sciences