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Expression of the Three Influenza Virus Polymerase Proteins in a Single Cell Allows Growth Complementation of Viral Mutants
Mark Krystal, Ruan Li, Douglas Lyles, George Pavlakis and Peter Palese
Proceedings of the National Academy of Sciences of the United States of America
Vol. 83, No. 8 (Apr. 15, 1986), pp. 2709-2713
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/27355
Page Count: 5
You can always find the topics here!Topics: Cell lines, Complementation, Viruses, RNA, Transformed cell line, NIH 3T3 cells, Orthomyxoviridae, Cell growth, 3T3 cells, Plasmids
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Transformed cell lines derived from murine C127 cells were constructed that express the influenza virus RNA-dependent RNA polymerase proteins (PA, PB1, and PB2). Cell lines that express only one or all three of the proteins were tested for their ability to complement temperature-sensitive viral mutants incubated at the nonpermissive temperature. Two cell lines were isolated that express all three polymerase genes and complement the growth of PB2 temperature-sensitive mutants at the nonpermissive temperature. One of these lines also complemented PA temperature-sensitive mutants. The viral titers obtained in these two cell lines were 12-fold to 1000-fold higher than the viral titers obtained upon growth of the corresponding temperature-sensitive mutant in C127 cells at the nonpermissive temperature.
Proceedings of the National Academy of Sciences of the United States of America © 1986 National Academy of Sciences