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The Chicken δ 1-Crystallin Gene Promoter: Binding of Transcription Factor(s) to the Upstream G+C-Rich Region is Necessary for Promoter Function in vitro
Gokul C. Das and Joram Piatigorsky
Proceedings of the National Academy of Sciences of the United States of America
Vol. 83, No. 10 (May 15, 1986), pp. 3131-3135
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/27455
Page Count: 5
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There are two linked δ -crystallin genes in the chicken (5′ δ 1-δ 2 3′). Only the δ 1 gene has been shown definitively to be active in the lens. Transcription of deletion mutants, reported here, shows that the sequences necessary for the functioning of the δ 1 promoter in a HeLa cell extract are located upstream from the RNA initiation site, between nucleotide positions -121 and -38. This region includes a number of G+C-rich motifs, including one hexanucleotide sequence, CCGCCC, that is repeated six times in the simian virus 40 (SV40) promoter. Competition experiments with purified fragments from the δ 1-crystallin gene promoter showed that binding of transcription factor(s) from the HeLa cell extract to this G+C-rich region is required for promoter activity in vitro. Further, competition experiments using three different fragments from the SV40 promoter suggest that the transcription factor(s) is similar to Sp1, which stimulates transcription by binding to the G+C-rich 21-base-pair repeats of the SV40 promoter, and differs from that which interacts with the SV40 enhancer region.
Proceedings of the National Academy of Sciences of the United States of America © 1986 National Academy of Sciences