You are not currently logged in.
Access your personal account or get JSTOR access through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Differentiation of Myeloid Cells is Accompanied by Increased Levels of pp60c-src Protein and Kinase Activity
Connie E. Gee, James Griffin, Leandro Sastre, Linda J. Miller, Timothy A. Springer, Helen Piwnica-Worms and Thomas M. Roberts
Proceedings of the National Academy of Sciences of the United States of America
Vol. 83, No. 14 (Jul. 15, 1986), pp. 5131-5135
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/27875
Page Count: 5
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
We have detected a significant increase in the levels of pp60c-src kinase activity associated with the differentiation of myeloid cell lines HL-60 and U-937. The induction of pp60c-src kinase activity becomes apparent ≈ 14 hr after the addition of phorbol 12-myristate 13-acetate and increases 20-fold by 72 hr. The enhanced kinase activity can be accounted for by elevated levels of c-src protein in the differentiated cells. When nonleukemic bone marrow cells were examined, myeloid progenitor cells exhibited a low level of pp60c-src kinase activity. As these cells are allowed to differentiate in culture, the resulting adherent monocytes are as high in pp60c-src kinase activity as HL-60 cells induced to differentiate into monocytes. A strong correlation is found between the levels of pp60c-src kinase activity and the degree of monocytic differentiation of the cells from patients with acute myeloid leukemia. Our findings suggest that the activation of pp60c-src kinase activity is a normal physiological event associated with myeloid differentiation.
Proceedings of the National Academy of Sciences of the United States of America © 1986 National Academy of Sciences