Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Designed oligomers of cyanovirin-N show enhanced HIV neutralization

Jennifer R. Keeffe, Priyanthi N. P. Gnanapragasam, Sarah K. Gillespie, John Yong, Pamela J. Bjorkman and Stephen L. Mayo
Proceedings of the National Academy of Sciences of the United States of America
Vol. 108, No. 34 (August 23, 2011), pp. 14079-14084
Stable URL: http://www.jstor.org/stable/27979464
Page Count: 6
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Preview not available

Abstract

Cyanovirin-N (CV-N) is a small, cyanobacterial lectin that neutralizes many enveloped viruses, including human immunodeficiency virus type I (HIV-1). This antiviral activity is attributed to two homologous carbohydrate binding sites that specifically bind high mannose glycosylation present on envelope glycoproteins such as HIV-1 gp120. We created obligate CV-N oligomers to determine whether increasing the number of binding sites has an effect on viral neutralization. A tandem repeat of two CV-N molecules (CVN2) increased HIV-1 neutralization activity by up to 18-fold compared to wild-type CV-N. In addition, the CVN2 variants showed extensive cross-clade reactivity and were often more potent than broadly neutralizing anti-HIV antibodies. The improvement in activity and broad cross-strain HIV neutralization exhibited by these molecules holds promise for the future therapeutic utility of these and other engineered CV-N variants.

Page Thumbnails

  • Thumbnail: Page 
14079
    14079
  • Thumbnail: Page 
14080
    14080
  • Thumbnail: Page 
14081
    14081
  • Thumbnail: Page 
14082
    14082
  • Thumbnail: Page 
14083
    14083
  • Thumbnail: Page 
14084
    14084