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The C-C (6-4) UV Photoproduct is Mutagenic in Escherichia coli
Barry W. Glickman, Roel M. Schaaper, William A. Haseltine, Ronnie L. Dunn and Douglas E. Brash
Proceedings of the National Academy of Sciences of the United States of America
Vol. 83, No. 18 (Sep. 15, 1986), pp. 6945-6949
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/28289
Page Count: 5
You can always find the topics here!Topics: Genetic mutation, Lesions, Dimers, Methylation, Mutagenesis, Pyrimidine dimers, Genetics, Sequencing, Nonsense mutation, DNA
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Mutation induced by ultraviolet light is predominantly targeted by UV photoproducts. Two primary candidates for the premutagenic lesion are the cyclobutane pyrimidine dimer and the less frequent (by a factor of 10) pyrimidine-pyrimidone (6-4) photoproduct. Methylation of the 3′-cytosine in the sequence 5′ CCAGG 3′ reduces the yield of (6-4) lesions, but not of cyclobutane dimers, at these sites. By taking advantage of mutants deficient in cytosine methylation, we show here that at the three sites in the lacI gene of Escherichia coli having this sequence, the specific increase in the formation of the (6-4) photoproducts is accompanied by a concomitant increase in mutation. At each site, a G· C to A· T transition results in an amber mutation. In the unmethylated state, these sites become among the most frequent nonsense mutations recovered. We conclude that the (6-4) photoproduct constitutes a major premutagenic lesion in E. coli.
Proceedings of the National Academy of Sciences of the United States of America © 1986 National Academy of Sciences