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Self-obsorption coefficient for 14C in Daphnia pulex ranging from 1.1 to 2.9 mm long were determined with a liquid scintillation counting procedure. Coefficients were calculated from two regressions: that of the radioactivity of labeled whole animals on size and that of the radioactivity of labeled animals digested for 24 hr at 60C in the solubilizer NCS on size. Self-absorption coefficients in creased from 1.23 for animals with a mean body carbon content of 6.6 /mu g C/ individual to 2.73 for animals with 44.7 /mu g C/ individual. If quenching is moderate and digestion complete, specific ativities of small animals can be determined directly using an efficient solubilizer, eliminating the need to use self-absorption coefficients.
Limnology and Oceanography