Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

A Liquid Scintillation Procedure for Determining the Effect of Size on Self-Absorption of 14C in Daphnia pulex1

F. J. Ward, Brian Wong and G. G. C. Robinson
Limnology and Oceanography
Vol. 15, No. 4 (Jul., 1970), pp. 648-652
Stable URL: http://www.jstor.org/stable/2833928
Page Count: 5
  • More info
  • Cite this Item
A Liquid Scintillation Procedure for Determining the Effect of Size on Self-Absorption of 14C in Daphnia pulex1
Preview not available

Abstract

Self-obsorption coefficient for 14C in Daphnia pulex ranging from 1.1 to 2.9 mm long were determined with a liquid scintillation counting procedure. Coefficients were calculated from two regressions: that of the radioactivity of labeled whole animals on size and that of the radioactivity of labeled animals digested for 24 hr at 60C in the solubilizer NCS on size. Self-absorption coefficients in creased from 1.23 for animals with a mean body carbon content of 6.6 /mu g C/ individual to 2.73 for animals with 44.7 /mu g C/ individual. If quenching is moderate and digestion complete, specific ativities of small animals can be determined directly using an efficient solubilizer, eliminating the need to use self-absorption coefficients.

Page Thumbnails

  • Thumbnail: Page 
648
    648
  • Thumbnail: Page 
649
    649
  • Thumbnail: Page 
650
    650
  • Thumbnail: Page 
651
    651
  • Thumbnail: Page 
652
    652