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Biologically Active Precursor for Transforming Growth Factor Type α , Released by Retrovirally Transformed Cells
Ronald A. Ignotz, Brenda Kelly, Roger J. Davis and Joan Massagué
Proceedings of the National Academy of Sciences of the United States of America
Vol. 83, No. 17 (Sep. 1, 1986), pp. 6307-6311
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/28346
Page Count: 5
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Retrovirus-transformed rat cells that actively express transforming growth factor type α (TGF-α ) release into the medium a soluble protein of 17-19 kDa that has been identified as a precursor for TGF-α . The identification of this protein as a TGF-α precursor is based on its recognition by specific antibodies and by the ability of elastase to convert this protein into a 6-kDa polypeptide with the properties of mature TGF-α . This TGF-α precursor binds to epidermal growth factor/TGF-α receptors and activates the receptor-associated tyrosine kinase activity in intact cells. The biological potency of this precursor is not markedly increased by conversion into mature TGF-α in vitro. These studies demonstrate the ability of transformed cells to release a TGF-α precursor capable of strong mitogenic action in vivo.
Proceedings of the National Academy of Sciences of the United States of America © 1986 National Academy of Sciences