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Interaction of a Common Factor with Conserved Promoter and Enhancer Sequences in Histone H2B, Immunoglobulin, and U2 Small Nuclear RNA (snRNA) Genes
Hazel L. Sive and Robert G. Roeder
Proceedings of the National Academy of Sciences of the United States of America
Vol. 83, No. 17 (Sep. 1, 1986), pp. 6382-6386
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/28361
Page Count: 5
You can always find the topics here!Topics: Genes, Small nuclear RNA, DNA, Plasmids, HeLa cells, Immunoglobulins, Consensus sequence, Nuclear interactions, Histones, Promoter regions
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We have examined the interaction of factors in HeLa cell nuclear extracts with a human histone H2B gene (H2B) promoter. Protein-DNA mobility-shift and DNase I protection assays detected a factor(s) binding to a 15-base-pair consensus element that is essential for efficient H2B transcription in vitro. Part of this consensus sequence is the octanucleotide ATTTGCAT, which is apparently a functional component of several non-histone genes. A subset of these genes, including a human U2 small nuclear RNA (snRNA) gene promoter, a mouse immunoglobulin heavy chain enhancer, and a mouse light chain promoter, were shown to interact with the H2B consensus sequence-binding factor(s). These results suggest that a common factor or closely related factors may contribute to the regulation of these and other genes that share the octanucleotide sequence.
Proceedings of the National Academy of Sciences of the United States of America © 1986 National Academy of Sciences