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Elevated Levels of mRNA Can Account for the Trans-Activation of Human Immunodeficiency Virus
B. Matija Peterlin, Paul A. Luciw, Philip J. Barr and Michael D. Walker
Proceedings of the National Academy of Sciences of the United States of America
Vol. 83, No. 24 (Dec. 15, 1986), pp. 9734-9738
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/28698
Page Count: 5
You can always find the topics here!Topics: HeLa cells, Transactivation, HIV, RNA, Plasmids, Messenger RNA, Cell lines, Genes, tat genes, Genomes
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The genome of human immunodeficiency virus encodes a protein that dramatically elevates amounts of viral proteins. The precise mechanism of this trans-activation remains to be established. It has been reported that trans-activation can occur without major changes in the levels of mRNA. We constructed recombinant plasmids containing those viral sequences required in cis for trans-activation linked to the chloramphenicol acetyltransferase gene. These plasmids were introduced into cultured cells in either the presence or absence of a second plasmid that directed expression of the viral trans-activator protein. Expression of the chloramphenicol acetyltransferase gene was measured at the level of protein (by enzymatic assay) and RNA (by ribonuclease protection and primer extension). Our results demonstrate that trans-activation is accompanied by large increases in mRNA levels; these increases may be sufficient to explain the elevated levels of trans-activated protein.
Proceedings of the National Academy of Sciences of the United States of America © 1986 National Academy of Sciences