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Molecular Cloning and Expression of a Complementary DNA for Inositol 1,4,5-Trisphosphate 3-Kinase

Kwan Yong Choi, Ha Kun Kim, Sang Yeol Lee, Kyung Ho Moon, Sang Soo Sim, Jae Won Kim, Hong Keun Chung and Sue Goo Rhee
Science
New Series, Vol. 248, No. 4951 (Apr. 6, 1990), pp. 64-66
Stable URL: http://www.jstor.org/stable/2874049
Page Count: 3
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Molecular Cloning and Expression of a Complementary DNA for Inositol 1,4,5-Trisphosphate 3-Kinase
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Abstract

A complementary DNA (cDNA) clone that encodes inositol 1,4,5-trisphosphate 3-kinase was isolated from a rat brain cDNA expression library with the use of monoclonal antibodies. This clone had an open reading frame that would direct the synthesis of a protein consisting of 449 amino acids and with a molecular mass of 49,853 daltons. The putative protein revealed a potential calmodulin-binding site and six regions with amino acid compositions (PEST regions) common to proteins that are susceptible to calpain. Expression of the cDNA in COS cells resulted in an approximately 150-fold increase in inositol 1,4,5-trisphosphate 3-kinase activity of these cells.

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