Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

ACh Receptor-Rich Membrane Domains Organized in Fibroblasts by Recombinant 43-Kildalton Protein

William D. Phillips, Carrie Kopta, Paul Blount, Paul D. Gardner, Joe Henry Steinbach and John Paul Merlie
Science
New Series, Vol. 251, No. 4993 (Feb. 1, 1991), pp. 568-570
Stable URL: http://www.jstor.org/stable/2875093
Page Count: 3
  • More info
  • Cite this Item
ACh Receptor-Rich Membrane Domains Organized in Fibroblasts by Recombinant 43-Kildalton Protein
Preview not available

Abstract

Neurotransmitter receptors are generally clustered in the postsynaptic membrane. The mechanism of clustering was analyzed with fibroblast cell lines that were stably transfected with the four subunits for fetal (α, β, γ, δ) or adult (α, β, ε, δ) type mouse muscle nicotinic acetylcholine receptors (AChRs). Immunofluorescent staining indicated that AChRs were dispersed on the surface of these cells. When transiently transfected with an expression construct encoding a 43-kilodalton protein that is normally concentrated under the postsynaptic membrane, AChRs expressed in these cells became aggregated in large cell-surface clusters, colocalized with the 43-kilodalton protein. This suggests that 43-kilodalton protein can induce AChR clustering and that cluster induction involves direct contact between AChR and 43-kilodalton protein.

Page Thumbnails

  • Thumbnail: Page 
568
    568
  • Thumbnail: Page 
569
    569
  • Thumbnail: Page 
570
    570