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Fatty Acid Biosynthesis Redirected to Medium Chains in Transgenic Oilseed Plants
Toni A. Voelker, Ann C. Worrell, Lana Anderson, Janice Bleibaum, Calvin Fan, Deborah J. Hawkins, Sharon E. Radke and H. Maelor Davies
New Series, Vol. 257, No. 5066 (Jul. 3, 1992), pp. 72-74
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/2877434
Page Count: 3
You can always find the topics here!Topics: Laurates, Bays, Plants, Complementary DNA, Tracheoesophageal fistula, Transgenic plants, Amino acids, Fatty acids, Lipids, Plastids
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Medium-chain fatty acids (FAs), found in storage lipids of certain plants, are an important renewable resource. Seeds of undomesticated California bay accumulate laurate (12:0), and a 12:0-acyl-carrier protein thioesterase (BTE) has been purified from this tissue. Sequencing of BTE enabled the cloning of a complementary DNA coding for a plastid-targeted preprotein. Expression of the complementary DNA in the seeds of Arabidopsis thaliana resulted in BTE activity, and medium chains accumulated at the expense of long-chain (≥ 16) FAs. Laurate became the most abundant FA species and was deposited in the storage triacylglycerols. These results demonstrate a mechanism for medium-chain FA synthesis in plants.
Science © 1992 American Association for the Advancement of Science