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Functional Importance of Sequence in the Stem-Loop of a Transcription Terminator
Sheau-Wei C. Cheng, Eileen C. Lynch, Kenneth R. Leason, Donald L. Court, Bruce A. Shapiro and David I. Friedman
New Series, Vol. 254, No. 5035 (Nov. 22, 1991), pp. 1205-1207
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/2879338
Page Count: 3
You can always find the topics here!Topics: RNA, Genetic mutation, Terminator regions, Dyadic relations, Plasmids, DNA, Nucleotides, Nucleotide sequences, Hybridity, Duplexes
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Intrinsic transcription terminators of prokaryotes are distinguished by a common RNA motif: a stem-loop structure high in guanine and cytosine content, followed by multiple uridine residues. Models explaining intrinsic terminators postulate that the stem-loop sequence is necessary only to form structure. In the tR2 terminator of coliphage λ, single-nucleotide changes reducing potential RNA stem stability eliminated tR2 activity, and a compensatory change that restored the stem structure restored terminator activity. However, multiple changes in the stem sequence that should have either maintained or increased stability reduced terminator activity. These results suggest that the ability of the stem-loop structure to signal transcription termination depends on sequence specificity and secondary structure.
Science © 1991 American Association for the Advancement of Science