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Cloning and Expression of a Cocaine-Sensitive Dopamine Transporter Complementary DNA

Shoichi Shimada, Shigeo Kitayama, Chien-Liang Lin, Amrat Patel, Elizabeth Nanthakumar, Paul Gregor, Michael Kuhar and George Uhl
Science
New Series, Vol. 254, No. 5031 (Oct. 25, 1991), pp. 576-578
Stable URL: http://www.jstor.org/stable/2879393
Page Count: 3
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Cloning and Expression of a Cocaine-Sensitive Dopamine Transporter Complementary DNA
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Abstract

A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and γ-aminobutyric acid transporters. The expressed complementary DNA confers transport of [$^3$H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [$^3$H]CFT {[$^3$H]2β-carbomethoxy-3β-(4-fluorophenyl)tropane} to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.

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