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Cloning of the 62-Kilodalton Component of Basic Transcription Factor BTF2

Laurent Fischer, Matthieu Gerard, Christian Chalut, Yves Lutz, Sandrine Humbert, Masamoto Kanno, Pierre Chambon and Jean-Marc Egly
Science
New Series, Vol. 257, No. 5075 (Sep. 4, 1992), pp. 1392-1395
Stable URL: http://www.jstor.org/stable/2879752
Page Count: 4
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Cloning of the 62-Kilodalton Component of Basic Transcription Factor BTF2
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Abstract

Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide inhibited transcription in vitro. Immunoaffinity experiments demonstrated that the 62-kilodalton component is closely associated with the other polypeptides present in the BTF2 factor. Sequence similarity suggests that BTF2 may be the human counterpart of RNA polymerase II initiation factor b from yeast.

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