Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Isolation of ORC6, a Component of the Yeast Origin Recognition Complex by a One-Hybrid System

Joachim J. Li and Ira Herskowitz
Science
New Series, Vol. 262, No. 5141 (Dec. 17, 1993), pp. 1870-1874
Stable URL: http://www.jstor.org/stable/2882828
Page Count: 5
  • More info
  • Cite this Item
Isolation of ORC6, a Component of the Yeast Origin Recognition Complex by a One-Hybrid System
Preview not available

Abstract

Here a method is described to identify genes encoding proteins that recognize a specific DNA sequence. A bank of random protein segments tagged with a transcriptional activation domain is screened for proteins that can activate a reporter gene containing the sequence in its promoter. This strategy was used to identify an essential protein that interacts in vivo with the yeast origin of DNA replication. Matches between its predicted amino acid sequence and peptide sequence obtained from the 50-kilodalton subunit of the yeast origin recognition complex (ORC) established that the gene isolated here, ORC6, encodes this subunit. These observations provide evidence that ORC recognizes yeast replication origins in vivo.

Page Thumbnails

  • Thumbnail: Page 
1870
    1870
  • Thumbnail: Page 
1871
    1871
  • Thumbnail: Page 
1872
    1872
  • Thumbnail: Page 
1873
    1873
  • Thumbnail: Page 
1874
    1874