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U1-Mediated Exon Definition Interactions between AT-Ac and GT-AG Introns

Qiang Wu and Adrian R. Krainer
Science
New Series, Vol. 274, No. 5289 (Nov. 8, 1996), pp. 1005-1008
Stable URL: http://www.jstor.org/stable/2891308
Page Count: 4
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U1-Mediated Exon Definition Interactions between AT-Ac and GT-AG Introns
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Abstract

A minor class of metazoan introns has well-conserved splice sites with 5′-AU-AC-3′ boundaries, compared to the 5′-GU-AG-3′ boundaries and degenerate splice sites of conventional introns. Splicing of the AT-AC intron 2 of a sodium channel (SCN4A) precursor messenger RNA in vitro did not require inhibition of conventional splicing and required adenosine triphosphate, magnesium, and U12 small nuclear RNA (snRNA) When exon 3 was followed by the 5′ splice site from the downstream conventional intron, splicing of intron 2 was greatly stimulated. This effect was U1 snRNA-dependent, unlike the basal AT-AC splicing reaction. Therefore, U1-mediated exon definition interactions can coordinate the activities of major and minor spliceosomes.

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