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Expression and Assembly of Functional Bacterial Luciferase in Plants
Csaba Koncz, Olof Olsson, William H. R. Langridge, Jeff Schell and Aladar A. Szalay
Proceedings of the National Academy of Sciences of the United States of America
Vol. 84, No. 1 (Jan. 1, 1987), pp. 131-135
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/28962
Page Count: 5
You can always find the topics here!Topics: Carrots, Plant cells, Protoplasts, Plasmids, Genes, Enzymes, DNA, Plant tissues, Electroporation, Cells
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The luxA and luxB structural genes of Vibrio harveyi luciferase [alkanal, reduced FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 126.96.36.199] were introduced into a plant expression vector and transferred into tobacco and carrot cells by Agrobacterium-mediated or direct DNA transformation. Simultaneous expression of the luxA and luxB genes was monitored by protein immunoblot analysis. Luciferase-mediated light emission provided evidence for the assembly of the two protein subunits into a functional dimeric enzyme in plant protoplasts, in transformed calli, and in leaves of transformed plants. Bacterial luciferase may provide a useful marker-gene system for the quantitative assay of coordinate gene expression in transgenic plants.
Proceedings of the National Academy of Sciences of the United States of America © 1987 National Academy of Sciences