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Expression and Assembly of Functional Bacterial Luciferase in Plants

Csaba Koncz, Olof Olsson, William H. R. Langridge, Jeff Schell and Aladar A. Szalay
Proceedings of the National Academy of Sciences of the United States of America
Vol. 84, No. 1 (Jan. 1, 1987), pp. 131-135
Stable URL: http://www.jstor.org/stable/28962
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Expression and Assembly of Functional Bacterial Luciferase in Plants
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Abstract

The luxA and luxB structural genes of Vibrio harveyi luciferase [alkanal, reduced FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 1.14.14.3] were introduced into a plant expression vector and transferred into tobacco and carrot cells by Agrobacterium-mediated or direct DNA transformation. Simultaneous expression of the luxA and luxB genes was monitored by protein immunoblot analysis. Luciferase-mediated light emission provided evidence for the assembly of the two protein subunits into a functional dimeric enzyme in plant protoplasts, in transformed calli, and in leaves of transformed plants. Bacterial luciferase may provide a useful marker-gene system for the quantitative assay of coordinate gene expression in transgenic plants.

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