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Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, Polη

Robert E. Johnson, Satya Prakash and Louise Prakash
Science
New Series, Vol. 283, No. 5404 (Feb. 12, 1999), pp. 1001-1004
Stable URL: http://www.jstor.org/stable/2897731
Page Count: 4
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Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, Polη
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Abstract

The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase η.

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