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Induction of Synthesis of the Cytolytic C9 (Ninth Component of Complement)-Related Protein in Human Peripheral Mononuclear Cells by Monoclonal Antibody OKT3 or Interleukin 2: Correlation with Cytotoxicity and Lymphocyte Phenotype
Dale E. Martin, Leora S. Zalman, Gundram Jung and Hans J. Muller-Eberhard
Proceedings of the National Academy of Sciences of the United States of America
Vol. 84, No. 9 (May 1, 1987), pp. 2946-2950
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/29302
Page Count: 5
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Synthesis of the cytolytic C9-related protein (C9RP) was induced by activation of resting human peripheral T lymphocytes with the anti-CD3 antibody OKT3 or interleukin 2. Comparison of cellular cytotoxicity and C9RP content at various times during activation yielded a coefficient of correlation r = 0.92. During OKT3 stimulation of peripheral mononuclear cells, maximal C9RP content and cytotoxicity were observed by day 2 or 3, with subsequent decline to baseline values by day 5, whereas during interleukin 2 stimulation, both parameters reached the maximal level at days 3-5. After fluorescence-activated cell sorting, C9RP and cytotoxicity were quantitated in CD4+, CD8+, and Leu-19+ subsets. In OKT3-activated CD8+ cells, C9RP increased to ≈ 3 × 106 molecules per cell, with a corresponding increase in lysis of human melanoma cells mediated by anti-CD3-anti-melanoma monoclonal antibody conjugates. Interleukin 2-stimulated CD8+ cells showed similar increases, but cytotoxicity was conjugate-independent. Activated CD4+ cells showed minimal increase in C9RP content. Leu-19+ cells, which exhibit natural killer cell activity, had a high C9RP content (≈ 2.5 × 106 molecules per cell) before stimulation.
Proceedings of the National Academy of Sciences of the United States of America © 1987 National Academy of Sciences