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Purification, Properties, and Immunocytochemical Localization of Human Liver Peroxisomal Enoyl-CoA Hydratase/3-hydroxyacyl-CoA Dehydrogenase
M. Kumudavalli Reddy, Nobuteru Usuda, M. Narahari Reddy, Edward R. Kuczmarski, M. Sambasiva Rao and Janardan K. Reddy
Proceedings of the National Academy of Sciences of the United States of America
Vol. 84, No. 10 (May 15, 1987), pp. 3214-3218
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/29363
Page Count: 5
You can always find the topics here!Topics: Enzymes, Liver, Amino acids, Peroxisomes, Antibodies, Dehydrogenases, Fatty acids, Rats, Molecular structure, Immunology
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A molecular understanding of genetic disease in which peroxisomal functions are impaired depends on analysis of the structure of normal and mutant enzymes of peroxisomes. We report experiments describing the isolation, characterization, and immunocytochemical localization of enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme (PBE) of the peroxisomal fatty acid β -oxidation system from normal human liver and compared it with that of rat liver enzyme. The human enzyme, purified ≈ 2300-fold by ion-exchange chromatography, is homogeneous as judged by NaDodSO4/PAGE. This PBE is localized exclusively in the matrix of peroxisomes in liver cells by the protein A/gold immunocytochemical method. The human PBE is similar to rat enzyme in size (Mr≈ 79,000), isoelectric point (pI, 9.8), pH optima, molecular structure as observed by rotary shadowing, and peptide pattern on NaDodSO4/PAGE after proteolytic digestion with Staphylococcus aureus V8 protease. The human and rat enzymes differed in their immunological properties by having partial identity with each other; this is reflected in their slightly dissimilar composition of the amino acids aspartic acid, threonine, glutamic acid, tyrosine, and glycine. COOH-terminal amino acids were similar for both the enzymes: -Gly-Ser-Leu-Ile-COOH. These results suggest that the human and rat liver PBE may be different in their amino acid sequences at their antigenic sites.
Proceedings of the National Academy of Sciences of the United States of America © 1987 National Academy of Sciences