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Isolation of a T-Cell Clone that Reacts with Both Antigen and Anti-Idiotype: Evidence for Anti-Idiotype as Internal Image for Antigen at the T-Cell Level
Rakesh Singhai and Julia G. Levy
Proceedings of the National Academy of Sciences of the United States of America
Vol. 84, No. 11 (Jun. 1, 1987), pp. 3836-3840
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/29494
Page Count: 5
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T-cell lines were derived from ferredoxin nonresponder B10.D2 mice that share an idiotype expressed by a monoclonal antibody (Fd-B2) with specificity for one of the two major antigenic determinants (the C determinant) of the antigen. The T-cell line and T-cell clones derived from it release interleukin 2 not only in the presence of anti-Fd-B2 idiotype antibody but in the presence of ferredoxin. The line was shown to be major histocompatibility complex-restricted in that it would respond to the anti-idiotype and antigen only in the context of presentation by cells of the H-2d haplotype. This observation also establishes that the nonresponder status of H-2d animals cannot be attributed to a lesion at the level of antigen presentation. Analysis of the fine specificity of one idiotypic clone showed that it responded only to the anti-idiotype or products of the antigen containing the C determinant, since enzymatically degraded peptides devoid of this determinant did not stimulate these cells. Furthermore, it was found that presentation of both the antigen and the anti-idiotype to the specific clone could be blocked by the Fd-B2 monoclonal antibody.
Proceedings of the National Academy of Sciences of the United States of America © 1987 National Academy of Sciences