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Effects of Electric Cell Fusion Treatment among Leaf Protoplasts of Populus Alba and Alnus Firma on Growth, Leaf Morphology, and RAPD Pattern of Eleven Acclimatized Plants
Hamako Sasamoto, Yohichi Wakita, Shinso Yokota, Nobuo Yoshizawa, Toshio Katsuki, Yoshihiko Nishiyama, Toshitaka Yokoyama and Mitsue Fukui
In Vitro Cellular & Developmental Biology. Plant
Vol. 42, No. 2 (Mar. - Apr., 2006), pp. 174-178
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/29736168
Page Count: 5
You can always find the topics here!Topics: Protoplasts, Plants, Cell fusion, Plant growth, Leaves, Plant morphology, Plant cells, Hybridity, Protoplast culture, Cell culture techniques
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This study reports the characterization of 11 plants regenerated from electrically fused protoplasts between Populus alba and Alnus firma. Growth characteristics of five regenerated plants (AP-1-AP-5) in terms of shoot height and leaf color showed small differences compared with those of P. alba grown in pots, and showed no difference in shoot height and diameter compared with those grown in nursery field. There was also no difference in the RAPD pattern between the plants regenerated from interfamilial protoplast fusion and P. alba. In contrast, the lately regenerated plants (AP-6-AP-11) grown in pots showed a marked difference in leaf morphology and RAPD pattern. There was a variation in the ratio of longitudinal to transverse length of leaves among the 11 plants from interfamilial fusions compared with that of protoclones and intraspecific fused protoplasts of P. alba.
In Vitro Cellular & Developmental Biology. Plant © 2006 Society for In Vitro Biology