You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Genome-Wide Identification of mRNAs Associated with the Translational Regulator PUMILIO in Drosophila melanogaster
André P. Gerber, Stefan Luschnig, Mark A. Krasnow, Patrick O. Brown and Daniel Herschlag
Proceedings of the National Academy of Sciences of the United States of America
Vol. 103, No. 12 (Mar. 21, 2006), pp. 4487-4492
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/30048968
Page Count: 6
You can always find the topics here!Topics: Messenger RNA, RNA, Embryos, Untranslated regions, Drosophila, Genes, Yeasts, Gene expression, RNA binding proteins, DNA
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Genome-wide identification of RNAs associated with RNA-binding proteins is crucial for deciphering posttranscriptional regulatory systems. PUMILIO is a member of the evolutionary conserved Puf-family of RNA-binding proteins that repress gene expression posttranscriptionally. We generated transgenic flies expressing affinity-tagged PUMILIO under the control of an ovary-specific promoter, and we purified PUMILIO from whole adult flies and embryos and analyzed associated mRNAs by using DNA microarrays. Distinct sets comprising hundreds of mRNAs were associated with PUMILIO at the two developmental stages. Many of these mRNAs encode functionally related proteins, supporting a model for coordinated regulation of posttranscriptional modules by specific RNA-binding proteins. We identified a characteristic sequence motif in the 3'-untranslated regions of mRNAs associated with PUMILIO, and the sufficiency of this motif for interaction with PUMILIO was confirmed by RNA pull-down experiments with biotinylated synthetic RNAs. The RNA motif strikingly resembles the one previously identified for Puf3p, one of five Saccharomyces cerevisiae Puf proteins; however, proteins encoded by the associated mRNAs in yeast and Drosophila do not appear to be related. The results suggest extensive posttranscriptional regulation by PUMILIO and uncover evolutionary features of this conserved family of RNA-binding proteins.
Proceedings of the National Academy of Sciences of the United States of America © 2006 National Academy of Sciences