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L1 Retrotransposition in Nondividing and Primary Human Somatic Cells
Shuji Kubo, Maria del Carmen Seleme, Harris S. Soifer, José Luis Garcia Perez, John V. Moran, Haig H. Kazazian Jr. and Noriyuki Kasahara
Proceedings of the National Academy of Sciences of the United States of America
Vol. 103, No. 21 (May 23, 2006), pp. 8036-8041
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/30049170
Page Count: 6
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Whether long interspersed element-1 (L1 or LINE-1) retrotransposition can occur in quiescent, nondividing, and/or terminally differentiated somatic cells has remained an unanswered fundamental question in human genetics. Here, we used a ubiquitously active phosphoglycerate kinase-1 promoter to drive the expression of a highly active human L1 element from an adenovirus-L1 hybrid vector. This vector system achieved retrotransposition in up to 91 % of actively growing immortalized cells, and we demonstrated that L1 retrotransposition can be suppressed by the reverse transcriptase inhibitor 3'-azido-3'-deoxythymidine. This adenovirus vector enabled efficient delivery of the L1 element into differentiated primary human somatic cells and G₁/S-arrested cells, resulting in retrotransposition in both cases; however, it was not detected in Go-arrested cells. Thus, these data indicate that L1 retrotransposition can occur in nondividing somatic cells.
Proceedings of the National Academy of Sciences of the United States of America © 2006 National Academy of Sciences