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Structural Basis for mRNA and tRNA Positioning on the Ribosome

Veysel Berk, Wen Zhang, Raj D. Pai and Jamie H. Doudna Cate
Proceedings of the National Academy of Sciences of the United States of America
Vol. 103, No. 43 (Oct. 24, 2006), pp. 15830-15834
Stable URL: http://www.jstor.org/stable/30052065
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Structural Basis for mRNA and tRNA Positioning on the Ribosome
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Abstract

Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-Å resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.

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