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Ubiquitin-Associated Domain of Mex67 Synchronizes Recruitment of the mRNA Export Machinery with Transcription
Carole Gwizdek, Nahid Iglesias, Manuel S. Rodriguez, Batool Ossareh-Nazari, Maria Hobeika, Gilles Divita, Françoise Stutz and Catherine Dargemont
Proceedings of the National Academy of Sciences of the United States of America
Vol. 103, No. 44 (Oct. 31, 2006), pp. 16376-16381
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/30052166
Page Count: 6
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The mRNA nuclear export receptor Mex67/Mtr2 is recruited to mRNAs through RNA-binding adaptors, including components of the THO/ TREX complex that couple transcription to mRNA export. Here we show that the ubiquitin-associated (UBA) domain of Mex67 is not only required for proper nuclear export of mRNA but also contributes to recruitment of Mex67 to transcribing genes. Our results reveal that the UBA domain of Mex67 directly interacts with polyubiquitin chains and with Hprl, a component of the THO/TREX complex, which is regulated by ubiquitylation in a transcription-dependent manner. This interaction transiently protects Hprl from ubiquitin/proteasome-mediated degradation and thereby coordinates recruitment of the mRNA export machinery with transcription and early messenger ribonucleoproteins assembly.
Proceedings of the National Academy of Sciences of the United States of America © 2006 National Academy of Sciences