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Quantification and Functional Analysis of Plasmacytoid Dendritic Cells in Patients with Chronic Hepatitis C Virus Infection
Nadège Goutagny, Claude Vieux, Evelyne Decullier, Benoît Ligeoix, Alberto Epstein, Christian Trépo, Patrice Couzigou, Geneviève Inchauspé and Christine Bain
The Journal of Infectious Diseases
Vol. 189, No. 9 (May 1, 2004), pp. 1646-1655
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30075807
Page Count: 10
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Background. Plasmacytoid dendritic cells (PDCs) are the major producers of interferon (IFN)-α within peripheral blood mononuclear cells (PBMCs). Methods. We analyzed whether chronic hepatitis C virus (HCV) infection could be linked to a defective function or number of PDCs. We evaluated the capacity of PBMCs from 5 cohorts of subjects to produce IFN-α after viral stimulation. We concomitantly analyzed the frequency of PDCs and the levels of IFN-α transcripts within the PBMCs from the same cohorts. Results. PBMCs from patients with chronic HCV infection receiving antiviral therapy displayed a reduced capacity to released IFN-α, compared with those from healthy individuals, those from long-term responders to therapy, and those from nontreated patients. This defect was significantly correlated with the percentage of PDCs. In addition, PDCs from patients with chronic HCV infection receiving therapy displayed a reduced intrinsic capacity to produce IFN-α, which could be linked to the level of IFN-α transcripts. Conclusion. Our obsevations point to an effect of the therapy on either the survival or the localization of PDCs, rather than a direct detrimental effect due to the viral infection during chronic HCV infection.
The Journal of Infectious Diseases © 2004 Oxford University Press