Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

HIV-1 Infection of Trophoblasts Is Independent of gp120/CD4 Interactions but Relies on Heparan Sulfate Proteoglycans

Gaël Vidricaire, Sonia Gauthier and Michel J. Tremblay
The Journal of Infectious Diseases
Vol. 195, No. 10 (May 15, 2007), pp. 1461-1471
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30088306
Page Count: 11
  • Download ($42.00)
  • Subscribe ($19.50)
  • Cite this Item
HIV-1 Infection of Trophoblasts Is Independent of gp120/CD4 Interactions but Relies on Heparan Sulfate Proteoglycans
Preview not available

Abstract

Mother-to-child transmission of human immunodeficiency virus type 1 (HIV-1) is the leading cause of HIV infection in infants. Direct infection of trophoblasts-cells forming the placental barrier-may cause this transmission. Entry of HIV-1 into trophoblasts is unusual for this retrovirus, because it is associated with endocytosis. However, given that trophoblasts express no or few receptors/coreceptors required for virus internalization, the mechanism underlying this event remains ambiguous. In the present study, we show that HIV-1 entry and infection of polarized trophoblasts are independent not only of CD4 but also of envelope (Env) glycoproteins gpl20 and gp41. Virus internalization, cytoplasmic release, reverse transcription, integration, and HIV-1 gene expression occurred with both fusion-incompetent and Env-deficient viruses. Importantly, fusion-independent infection was observed when we used viruses produced in a natural cellular reservoir (i.e., primary human cells). Finally, HIV-1 requires heparan sulfate proteoglycans for uptake in trophoblasts. Together, our findings illustrate that HIV-1 utilizes an unusual pathway for entering human polarized trophoblasts.

Page Thumbnails

  • Thumbnail: Page 
1461
    1461
  • Thumbnail: Page 
1462
    1462
  • Thumbnail: Page 
1463
    1463
  • Thumbnail: Page 
1464
    1464
  • Thumbnail: Page 
1465
    1465
  • Thumbnail: Page 
1466
    1466
  • Thumbnail: Page 
1467
    1467
  • Thumbnail: Page 
1468
    1468
  • Thumbnail: Page 
[1469]
    [1469]
  • Thumbnail: Page 
1470
    1470
  • Thumbnail: Page 
1471
    1471