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A Study in Culturing Tubercle Bacilli from Sputum
E. S. King and Margaret Lucas
The Journal of Infectious Diseases
Vol. 67, No. 3 (Nov. - Dec., 1940), pp. 213-216
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30092209
Page Count: 4
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This study was undertaken to determine from examination of several mediums which one resulted in the greatest number of positive cultures of tubercle bacilli when made from microscopically positive sputum specimens. A group of 15 mediums were used and 50 specimens of positive sputum were examined. Of these specimens 82% were positive for growth on our bean-egg medium containing 1.5% glycerine and 1:10,000 gentian violet after 21 days' incubation at 37 C. On the basis of the number of specimens cultured it proved definitely superior to any other medium. Both cultural methods and guinea pig inoculations should be employed on all specimens wherever possible since neither one alone seems to be 100% reliable.
The Journal of Infectious Diseases © 1940 Oxford University Press