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Staphylococcal Enterotoxin: Production Methods
M. J. Surgalla, J. L. Kadavy, M. S. Bergdoll and G. M. Dack
The Journal of Infectious Diseases
Vol. 89, No. 2 (Sep. - Oct., 1951), pp. 180-184
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30093985
Page Count: 5
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Three methods have been employed for producing large volumes of Staphylococcus enterotoxin in hydrolyzed casein mediums. These are: 1. Shallow cultures.—Fifty Roux bottles, each containing 200 ml of culture, are incubated at 37 C in a sealed incubator containing 20% carbon dioxide and 80% air. 2. Deep cultures in turning bottles.-— A sealed 12-gallon bottle containing 10 L of culture under 20% carbon dioxide plus 80% air is turned continuously in a horizontal position on rollers during incubation at 37 C. 3. Deep aerated cultures.—One to 15 L of deep culture is aerated with either air or a 20% carbon dioxide plus 80% oxygen gas mixture during incubation at 37 C.
The Journal of Infectious Diseases © 1951 Oxford University Press