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Studies on Immunity to Staphylococcal Infection in Mice: III. A Protein Antigen That Induces Protection against Challenge with Saline-Suspended Organisms

Richard D. Ekstedt
The Journal of Infectious Diseases
Vol. 113, No. 2 (Sep. - Oct., 1963), pp. 105-109
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30099470
Page Count: 5
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Studies on Immunity to Staphylococcal Infection in Mice: III. A Protein Antigen That Induces Protection against Challenge with Saline-Suspended Organisms
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Abstract

Whole heat-killed cells of Staphylococcus aureus, Smith diffuse strain, grown in trypsin lost their ability to stimulate protective antibody in mice challenged 2 weeks postvaccination with a lethal dose of the homologous organisms in saline suspension. Animals immunized in the same manner but challenged with a lethal dose of the homologous organisms in 5% mucin showed a high degree of resistance. Nontrypsinized killed vaccines induced protection against both forms of challenge. A crude ammonium sulfate fraction prepared from Mickle-disintegrated soluble cell substance was also shown to induce protection to challenge with both mucin- and saline-suspended organisms. Boiling or trypsin digestion of the ammonium sulfate fraction destroyed its immunogenicity for mice when the animals were challenged with the organisms in saline suspension but did not affect its activity in the mucin challenge system. The immunogenically active fraction from 5. aureus, Smith strain, showed a typical ultraviolet absorption curve with approximately 20% nucleic acid. It also contained about 0.63% hexoses as determined by the anthrone test. It had an isoelectric point at pH 5.5 to 5.6, and the electrophoretic pattern showed 3 components.

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