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Histochemical Demonstration of Changes in Enzymatic Activity of KB Cells Produced by Staphylococcal Alpha and Beta Hemolysins
J. Jeljaszewicz, S. Szmigielski, M. Korbecki and C. Zak
The Journal of Infectious Diseases
Vol. 115, No. 5 (Dec., 1965), pp. 421-428
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30102037
Page Count: 8
You can always find the topics here!Topics: KB cells, Toxins, Hemolysin proteins, Dosage, Enzymes, Acetates, Phosphatases, Cell culture techniques, Cell lines, Lipid metabolism
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Standardized suspensions of KB cells were incubated for 6 hours at 37 C with various doses of purified staphylococcal α and β-hemolysins and then were observed for hematoxylin and Sudan B staining. Histochemical reactions for the following enzymes were performed thiamine pyrophosphatase, γ-glutamyl transpeptidase, acid and alkaline phosphatases, adenosine triphosphatase, 5'nucleotidase, β-naphthyl acetate and 5-bromo-indoxyl acetate esterases. Enzyme activity was expressed in percentages of cells exhibiting the presence of typical granulations. Different disturbances in enzymatic activities by each toxin were noted. Alpha hemolysin caused significant changes in 5'-nucleotidase activity. Beta hemolysin resulted in cellular changes suggesting involvement of the lipid metabolism. Some implications of these results are discussed.
The Journal of Infectious Diseases © 1965 Oxford University Press