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Host-Parasite Relationships in Brucellosis: III. Behavior of Avirulent Brucella in Tissue Culture Monocytes
Bob A. Freeman, Gary R. Pearson and William D. Hines
The Journal of Infectious Diseases
Vol. 114, No. 5 (Dec., 1964), pp. 441-449
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30102057
Page Count: 9
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Growth of brucella in guinea pig mononuclear phagocytes has been investigated in order to determine the mechanism of avirulence. With several experimental approaches it has been shown that rough, avirulent brucella grow intracellularly in tissue culture. These approaches have included demonstrations of intracellular growth of avirulent brucella by plate count methods, provided bacteriostatic agents are used in the tissue culture medium to control extracellular growth. Growth of avirulent brucella in host cells is shown by direct microscopic enumeration of intracellular bacteria. When monocyte cultures are infected with a mixed inoculum of virulent and avirulent brucella, the avirulent brucella cause the death of the host cell with the result that all freed brucella, smooth and rough alike, are killed by the streptomycin in the medium. It is proposed that in the brucella-guinea pig monocyte system the apparent failure of avirulent brucella to multiply can be explained by the destructive effect of the parasite on the host cell and its subsequent killing by antibiotic in the medium and is not based on the inability of avirulent brucella to multiply in their intracellular environment. The factors involved in the destruction of macrophage by avirulent brucella are unknown.
The Journal of Infectious Diseases © 1964 Oxford University Press