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Detection of an Adherence Factor of Enteropathogenic Escherichia coli with a DNA Probe
James P. Nataro, Mary M. Baldini, James B. Kaper, Robert E. Black, Nora Bravo and Myron M. Levine
The Journal of Infectious Diseases
Vol. 152, No. 3 (Sep., 1985), pp. 560-565
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30105313
Page Count: 6
You can always find the topics here!Topics: Diarrhea, Infants, Escherichia coli, Plasmids, DNA probes, Epidemiology, HeLa cells, Children, Bacteria, Pathogenesis
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A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coli isolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF]) was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.
The Journal of Infectious Diseases © 1985 Oxford University Press