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Human Immune Responses to a Novel Norwalk Virus Vaccine Delivered in Transgenic Potatoes

Carol O. Tacket, Hugh S. Mason, Genevieve Losonsky, Mary K. Estes, Myron M. Levine and Charles J. Arntzen
The Journal of Infectious Diseases
Vol. 182, No. 1 (Jul., 2000), pp. 302-305
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30110046
Page Count: 4
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Human Immune Responses to a Novel Norwalk Virus Vaccine Delivered in Transgenic Potatoes
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Abstract

A new approach for delivering vaccine antigens is the use of inexpensive, plentiful, plantbased oral vaccines. Norwalk virus capsid protein (NVCP), assembled into virus-like particles, was used as a test antigen, to determine whether immune responses could be generated in volunteers who ingested transgenic potatoes. Twenty-four healthy adult volunteers received 2 or 3 doses of transgenic potato (n = 20) or 3 doses of wild-type potato (n = 4). Each dose consisted of 150 g of raw, peeled, diced potato that contained 215-751 µg of NVCP. Nineteen (95%) of 20 volunteers who ingested transgenic potatoes developed significant increases in the numbers of specific IgA antibody-secreting cells. Four (20%) of 20 volunteers developed specific serum IgG, and 6 (30%) of 20 volunteers developed specific stool IgA. Overall, 19 of 20 volunteers developed an immune response of some kind, although the level of serum antibody increases was modest.

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