Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Active Immunization with a Detoxified Escherichia coli J5 Lipopolysaccharide Group B Meningococcal Outer Membrane Protein Complex Vaccine Protects Animals from Experimental Sepsis

Alan S. Cross, Steven M. Opal, H. Shaw Warren, John E. Palardy, Kathleen Glaser, Nicolas A. Parejo and Apurba K. Bhattacharjee
The Journal of Infectious Diseases
Vol. 183, No. 7 (Apr. 1, 2001), pp. 1079-1086
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30111165
Page Count: 8
  • Download ($42.00)
  • Subscribe ($19.50)
  • Cite this Item
Active Immunization with a Detoxified Escherichia coli J5 Lipopolysaccharide Group B Meningococcal Outer Membrane Protein Complex Vaccine Protects Animals from Experimental Sepsis
Preview not available

Abstract

The passive infusion of antibodies elicited in rabbits with a detoxified J5 lipopolysaccharide (LPS)/group B meningococcal outer membrane protein complex vaccine protected neutropenic rats from heterologous lethal gram-negative bacterial infection. In this study, active immunization was studied in neutropenic rats infected with Pseudomonas aeruginosa, in the presence or absence of ceftazidime therapy, and with Klebsiella pneumoniae. This vaccine elicited a >200-fold increase in anti-J5 LPS antibody, which remained elevated throughout the duration of cyclophosphamide-induced neutropenia and for ≤3 months. There was improved survival among immunized versus control animals: 48% (13128) versus 7% (2/29) in Pseudomonas-challenged rats; 61% (11/18) versus 0% (0/10) in Pseudomonas- and ceftazdime-treated rats; and 64% (9/14) versus 13% (2115) in Klebsiella-challenged rats (P < .01 for each comparison). Immunized animals had lower levels of bacteria in organs and lower levels of circulating endotoxin at the onset of fever. In conclusion, active immunization with an anti-endotoxin vaccine improved survival after infection with ≥2 heterologous, clinically relevant bacterial species in immunocompromised animals. Active immunization with this vaccine merits further investigation.

Page Thumbnails

  • Thumbnail: Page 
1079
    1079
  • Thumbnail: Page 
1080
    1080
  • Thumbnail: Page 
1081
    1081
  • Thumbnail: Page 
1082
    1082
  • Thumbnail: Page 
1083
    1083
  • Thumbnail: Page 
1084
    1084
  • Thumbnail: Page 
1085
    1085
  • Thumbnail: Page 
1086
    1086