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Identification of a 65-kDa Mannoprotein as a Main Target of Human Cell-Mediated Immune Response to Candida Albicans

Antonella Torosantucci, Carla Bromuro, Maria J. Gomez, Clara M. Ausiello, Francesca Urbani and Antonio Cassone
The Journal of Infectious Diseases
Vol. 168, No. 2 (Aug., 1993), pp. 427-435
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30113145
Page Count: 9
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Identification of a 65-kDa Mannoprotein as a Main Target of Human Cell-Mediated Immune Response to Candida Albicans
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Abstract

To identify molecular targets of anticandidal cell-mediated immunity (CMI) in humans, a highly immunogenic mannoprotein fraction (MP-F2) of Candida albicans was studied. SDS- PAGE and gel-permeation chromatography separated MP-F2 into polydisperse mannoproteins of > 200-31.5 kDa. However, only a 65-kDa constituent specifically induced proliferation of human peripheral blood mononuclear cells (PBMC). Lymphoproliferation was accompanied by production of interleukin (IL)-lβ, interferon-γ, and IL-6 but not IL-4. MP-F2- and MP-65-induced PBMC proliferation was inhibited by an antagonist anti-T cell receptor antibody. Neither the purified protein derivative of Mycobacterium tuberculosis nor MP-65 activated naive lymphocytes from umbilical cord blood, although these cells proliferated extensively in response to both phytohemagglutinin and IL-2. These data strongly suggest that MP-65 is an immunodominant mannoprotein antigen that is ordinarily expressed as a target of anti-Candida CMI in healthy humans.

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