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Role of Cell Wall Polysaccharide in the Assessment of IgG Antibodies to the Capsular Polysaccharides of Streptococcus pneumoniae in Childhood

David Goldblatt, Roland J. Levinsky and Malcolm W. Turner
The Journal of Infectious Diseases
Vol. 166, No. 3 (Sep., 1992), pp. 632-634
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30113335
Page Count: 3
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Role of Cell Wall Polysaccharide in the Assessment of IgG Antibodies to the Capsular Polysaccharides of Streptococcus pneumoniae in Childhood
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Abstract

The interference of antibodies to pneumococcal cell wall polysaccharide (CWPS) in the measurement of antibodies to capsular polysaccharides in children was assessed after vaccination with pneumococcal polysaccharide vaccine. ELISAs were developed to measure IgG subclasses specific for pneumococcal types 3, 6, 19, and 23 and CWPS. Analysis of antibody levels to all four capsular polysaccharides was affected by the presence of antibodies to CWPS, and their removal altered both anti-capsular polysaccharide antibody levels and the interpretation of responses to the vaccine. Thus, it is likely that CWPS contaminating pure capsular polysaccharide reagents used in most standard immunoassays is responsible for falsely elevated measurements of antibodies to capsular polysaccharide and the incorrect assessment of anti-pneumococcal antibody status in childhood.

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