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Distinct Functional Activities in Canine Septic Shock of Monoclonal Antibodies Specific for the O Polysaccharide and Core Regions of Escherichia coli Lipopolysaccharide

William D. Hoffman, Matthew Pollack, Steven M. Banks, Lutchezar A. Koev, Michael A. Solomon, Robert L. Danner, Nancy Koles, Gretchen Guelde, Ido Yatsiv, Tamarya Mouginis, Ronald J. Elin, Jeanette M. Hosseini, John Bacher, Jennifer C. Porter and Charles Natanson
The Journal of Infectious Diseases
Vol. 169, No. 3 (Mar., 1994), pp. 553-561
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30113776
Page Count: 9
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Distinct Functional Activities in Canine Septic Shock of Monoclonal Antibodies Specific for the O Polysaccharide and Core Regions of Escherichia coli Lipopolysaccharide
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Abstract

Monoclonal antibodies (MAbs) specific for O polysaccharide or core oligosaccharide/lipid A of Escherichia coli O111:B4 lipopolysaccharide (LPS) were compared in canine septic shock. Animals received O-specific, core-specific, or control murine IgG2a MAbs (or saline) before intraperitoneal implantation of an E. coli O111:B4-infected clot. Animals were further random?? ized to ceftriaxone or saline. O-specific MAb significantly reduced bacteremia and endotoxemia but not serum tumor necrosis factor. Core-specific MAb significantly increased mean arterial pressure from day 4 to 28 (P = .02). In dogs not receiving ceftriaxone, survival was enhanced by O-specific MAb (4/5) compared with core-specific MAb (0/5) and control (1/8) (P = .03). Survival rates were similar (P = .22) but survival was prolonged in antibiotic-treated animals also receiving O-specific MAb (P = .02 vs. core-specific MAb and controls) or core-specific MAb (P = .08 vs. controls). These data support the complex role of LPS in sepsis and the discrete functional effects of MAbs specific for different elements of LPS.

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