Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Monitoring of Human Cytomegalovirus Infections in Pediatric Bone Marrow Transplant Recipients by Nucleic Acid Sequence-Based Amplification

Toshiya Aono, Kazuhiro Kondo, Hiroko Miyoshi, Keiko Tanaka-Taya, Motohiro Kondo, Yuko Osugi, Junichi Hara, Shintaro Okada and Koichi Yamanishi
The Journal of Infectious Diseases
Vol. 178, No. 5 (Nov., 1998), pp. 1244-1249
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30117347
Page Count: 6
  • Download ($42.00)
  • Subscribe ($19.50)
  • Cite this Item
Monitoring of Human Cytomegalovirus Infections in Pediatric Bone Marrow Transplant Recipients by Nucleic Acid Sequence-Based Amplification
Preview not available

Abstract

In the diagnosis of human cytomegalovirus (HCMV) infection, it is very important to distinguish symptomatic from asymptomatic infection. The nucleic acid sequence-based amplification (NASBA) technique was compared with single and nested polymerase chain reaction (PCR) methods. For NASBA detection, the $\beta_2.7$ transcript was chosen as a target because of its abundant active HCMV-specific expression. Of 20 pediatric bone marrow transplant (BMT) recipients, 8 developed HCMV-related clinical symptoms. The clinical sensitivities and specificities were 50% and 100% for single PCR, 100% and 67% for nested PCR, and 100% and 83% for NASBA, respectively. Follow-up of HCMV infections in pediatric BMT recipients showed that NASBA could both detect viral transcript prior to the onset of clinical symptoms and reflect clinical improvement due to antiviral therapy. These data suggest that NASBA should be useful for both predicting HCMV disease development and monitoring the effect of antiviral therapy.

Page Thumbnails

  • Thumbnail: Page 
1244
    1244
  • Thumbnail: Page 
1245
    1245
  • Thumbnail: Page 
1246
    1246
  • Thumbnail: Page 
1247
    1247
  • Thumbnail: Page 
1248
    1248
  • Thumbnail: Page 
1249
    1249