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Immunization of Mice with Antibiotic-Treated Escherichia coli Results in Enhanced Protection against Challenge with Homologous and Heterologous Bacteria

Giammarco Raponi, Nathan Keller, Berry P. Overbeek, Maja Rozenberg-Arska, Ruurd Torensma and Jan Verhoef
The Journal of Infectious Diseases
Vol. 163, No. 1 (Jan., 1991), pp. 122-127
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30119520
Page Count: 6
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Immunization of Mice with Antibiotic-Treated Escherichia coli Results in Enhanced Protection against Challenge with Homologous and Heterologous Bacteria
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Abstract

The murine immune response to Escherichia coli exposed to subminimal inhibitory concentrations of four antibiotics was investigated. Groups of mice were injected for 8 weeks with formalinkilled bacteria and subsequently challenged with 10 x LD50 of viable E. coli. Mice receiving saline only (controls) died within 24 h. The mortality of mice immunized with ciprofloxacintreated E. coli was significantly lower than that of mice immunized with E. coli untreated or treated with other antibiotics. Sera from mice immunized with ciprofloxacin-treated bacteria showed better bacteriostatic capacity and enhanced production of antibodies that bound to homologous and heterologous lipopolysaccharide isolated from several smooth and rough gram-negative strains. The better protection observed in mice immunized with ciprofloxacin-treated E. coli was probably due to an enhanced production of antibodies to epitopes on lipopolysaccharide that became better exposed and so more accessible after treatment with ciprofloxacin.

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