Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Antiborrelial Activity of Serum from Rats Injected with the Lyme Disease Spirochete

Charles S. Pavia, Vincent Kissel, Susan Bittker, Felipe Cabello and Seymour Levine
The Journal of Infectious Diseases
Vol. 163, No. 3 (Mar., 1991), pp. 656-659
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30123186
Page Count: 4
  • Download ($42.00)
  • Subscribe ($19.50)
  • Cite this Item
Antiborrelial Activity of Serum from Rats Injected with the Lyme Disease Spirochete
Preview not available

Abstract

The functional properties of humoral factors generated in rats immunized against Borrelia burgdorferi were investigated. After Lewis strain rats were injected intraperitoneally with live B. burgdorferi or in the footpad with dead borreliae incorporated in complete Freund's adjuvant, they produced high-titered antisera. At a dilution of ≤1:10, sera from immunized or infected rats but not control sera inhibited growth of B. burgdorferi in vitro. Neutralization of growth of three different strains of B. burgdorferi by posttreatment sera was dose-dependent and was detected equally well by direct microscopic counts or by measuring incorporation of tritiated adenine into newly synthesized nucleic acids. These findings provide direct evidence that infection or immunization with the Lyme disease spirochete induces the formation of serum factors capable of preventing the growth of B. burgdorferi in vitro.

Page Thumbnails

  • Thumbnail: Page 
656
    656
  • Thumbnail: Page 
657
    657
  • Thumbnail: Page 
658
    658
  • Thumbnail: Page 
659
    659