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Mechanisms for Mucosal Immunogenicity and Adjuvancy of Escherichia coli Labile Enterotoxin

Ichiro Takahashi, Mariarosaria Marinaro, Hiroshi Kiyono, Raymond J. Jackson, Ichiro Nakagawa, Kohtaro Fujihashi, Shigeyuki Hamada, John D. Clements, Kenneth L. Bost and Jerry R. McGhee
The Journal of Infectious Diseases
Vol. 173, No. 3 (Mar., 1996), pp. 627-635
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30126182
Page Count: 9
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Mechanisms for Mucosal Immunogenicity and Adjuvancy of Escherichia coli Labile Enterotoxin
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Abstract

Escherichia coli labile toxin (LT) was assessed as mucosal immunogen and as adjuvant for tetanus toxoid (TT) in mice. After oral administration of LT, C57BL/6 ($H-2^b$) and BALB/c ($H-2^d$) mice were high mucosal and serum antibody responders, while C3HZHeN ($H-2^k$) mice were low responders. High responders exhibited mainly serum IgG (including IgGl, IgG2a, and IgG2b), as well as IgM and IgA, while mucosal responses were IgA. Analysis of LT-B-specific CD4⁺ T helper (Th) cells from Peyer's patches (PP) or from spleen revealed a mixed Th1 (interferon-γ) and Th2 (interleukin4 and -5) cell pattern. Oral LT given with TT induced TT-specific response patterns identical to LT-B. Analysis of mRNA from TT-specific PP CD4⁺ Th cells also revealed a mixed Th1and Th2type response. Thus, antibody response profiles induced by LT are regulated by both CD4⁺ Th1 and Th2 cell types.

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