Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Live Subgroup B Respiratory Syncytial Virus Vaccines That Are Attenuated, Genetically Stable, and Immunogenic in Rodents and Nonhuman Primates

James E. Crowe Jr., Phuong T. Bui, Cai-Yen Firestone, Mark Connors, William R. Elkins, Robert M. Chanock and Brian R. Murphy
The Journal of Infectious Diseases
Vol. 173, No. 4 (Apr., 1996), pp. 829-839
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30132585
Page Count: 11
  • Download ($42.00)
  • Subscribe ($19.50)
  • Cite this Item
Live Subgroup B Respiratory Syncytial Virus Vaccines That Are Attenuated, Genetically Stable, and Immunogenic in Rodents and Nonhuman Primates
Preview not available

Abstract

Optimal immunization of neonates against disease caused by respiratory syncytial virus (RSV) probably will require multiple doses of a vaccine containing viruses of both subgroups A and B. Live subgroup B RSV mutants were generated containing multiple attenuating mutations, ts (temperature-sensitive) and non-ts (host range), that were introduced by prolonged passage in cell culture or by chemical mutagenesis. The cold-passaged (cp)-52 mutant was restricted in replication compared to wild type virus in rodents and nonhuman primates. In addition, the attenuation phenotype of cp-52 was stable after prolonged replication in immunosuppressed rodents. One or two ts mutations were then introduced into the cp-52 mutant to generate additional candidate vaccine strains that were more attenuated in vivo than the cp-52 parental virus. Tests in humans are being done to determine if one or more of the RSV B-l mutants exhibit a satisfactory balance between attenuation and immunogenicity.

Page Thumbnails

  • Thumbnail: Page 
829
    829
  • Thumbnail: Page 
830
    830
  • Thumbnail: Page 
[831]
    [831]
  • Thumbnail: Page 
832
    832
  • Thumbnail: Page 
833
    833
  • Thumbnail: Page 
834
    834
  • Thumbnail: Page 
835
    835
  • Thumbnail: Page 
836
    836
  • Thumbnail: Page 
837
    837
  • Thumbnail: Page 
838
    838
  • Thumbnail: Page 
839
    839